Fachbereich Biologie, Chemie, Pharmazie – Institut für Chemie und Biochemie Musculoskeletal Development and Regeneration GroupPhD student (m/f/d) 65%-part-time job Limited to 3 years Entgeltgruppe 13 TV-L FU Refence code: Adipo_2020 Bewerbungsende: 26.10.2020 Free University of Berlin, Germany

Fachbereich Biologie, Chemie, Pharmazie – Institut für Chemie und Biochemie Musculoskeletal Development and Regeneration Group PhD student

(m/f/d) 65%-part-time job Limited to 3 years Entgeltgruppe 13 TV-L FU Refence code: Adipo_2020 Bewerbungsende: 26.10.2020

Free University of Berlin, Germany

PhD student position is available in the Musculoskeletal Development and Regeneration Lab (https://www.bcp.fu-berlin.de/en/chemie/biochemie/research-groups/stricker-group/index.html) at the Institute of Chemistry and Biochemistry of the Freie Universität Berlin. We are a dynamic young group interested in transcriptional mechanisms controlling embryonic mesenchymal progenitor- and adult mesenchymal stem cell differentiation. The position will be part of a DFG-funded project addressing transcriptional and epigenetic mechanisms of adipogenic differentiation in development and the adult. We use cell models in combination with, where appropriate, mouse models.
Our lab is part of the vibrant interdisciplinary scientific landscape of Berlin with its unique collaborative environment. We are part of the ECRT (Einstein Center for Regenerative Therapies), BSRT (Berlin-Brandenburg School for Regenerative Therapies) and MyoGrad (International Research Training Group for Myology). The PhD project can be integrated into one of several Berlin-based graduate training schools that offer widespread education and support.

Job description:
Your task will be to conduct and develop a research project in close feedback with the lab’s PI. Methods that will be used include: FACS-Extraction of primary cells from mice for analysis and cultivation; Establishment and maintenance of primary cells and cell lines; Establishment of explant cultures from mouse embryos; transfection and siRNA mediated knockdown in cell lines; Analysis of cell lines and mouse tissues by RNA-Seq, ChIP-Seq and ATAC-Seq; Bioinformatic evaluation of sequencing data; Analysis of genetically modified mouse models (histology, RNA-in-situ hybridisation; immunolabeling); FACS analysis of adult stromal cells; Planning, application for, and implementation of work on transgenic mouse models. You will be able to present your work in national and international conferences, and are expected to contribute to manuscript and grant application development.

Requirements:
Master’s degree in Biochemistry, Biology or related disciplines.

Desirable:
Desired skills:

Good communication skills are prerequisite to work in our international team.

Experience in molecular biology, cell biology, and in transcriptional and epigenetic regulation – both practical and theoretical.
Basic knowledge in the area of mesenchymal cell differentiation in the embryo, especially myogenesis and adipogenesis, is an advantage; also basic knowledge of the anatomy and histology of the mouse embryo.
Experience in next-generation sequencing-based methods (RNA-Seq, ChIP-Seq, ATAC-Seq) as well as bioinformatic evaluation and interpretation of the data, also with the help of online databases.
Experience in the application of flow cytometry and fluorescence microscopy including confocal microscopy, as well as in programs for the evaluation of image data.
Experience in cell culture, especially with primary cells and tissue explants; application of cell differentiation assays; isolation of stroma cells from adipose tissue; knowledge of organoid models would be an advantage.
Experience and knowledge – both practical and theoretical – in dealing with transgenic mouse models, including Cre/loxP-based lineage tracing models, and the willingness to work with them, is particularly desirable. Practical experience in the analysis of mouse models using histological methods (frozen sections, histological staining), as well as using RNA-in-situ hybridization and immunostaining on tissue sections are advantageous. Knowledge of planning and applying for animal experiments as well as being in possession of a FELASA-B certificate are an advantage.
Additional qualifications: successful acquisition of third-party funding; proof of scientific manuscripts; capacity for teamwork and cooperation; good presentation and writing skills; experience in the supervision of bachelor or master students.

For further information, please contact Prof. Dr. Sigmar Stricker (sigmar.stricker@fu-berlin.de / +49 30 838 75799).

 

All application quoting the reference code should be addressed as an e-mail to Prof. Dr. Sigmar Stricker: sigmar.stricker@fu-berlin.de or postal to

Freie Universität Berlin

Fachbereich Biologie, Chemie, Pharmazie

Institut für Chemie und Biochemie

Musculoskeletal Development and Regeneration Group

Herrn Prof. Dr. Sigmar Stricker

Thielallee 63

14195 Berlin (Dahlem)

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click here to apply:- https://www.fu-berlin.de/universitaet/beruf-karriere/jobs/english/BC-Adipo_2020-E.html

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